CoViD TESTS

INSTRUMENTS

(Note: Whatever written in italics is absolute high yield points for any competitive exam)

🎯 RT-PCR (QuantStudio 5) :

       Use: mainly for CoViD

       Principle:  RNA => DNA

     On the basis of (viral load) CT i.e.Cycle Threshold, we tell that sample is Positive or Negative. 

💉Benefits: 

👉At a time we can check 94 sample 

👉Sensitivity is good. 

💉Drawbacks:

 Test nearly takes 1 hr 40 minutes for the result.

Graph during RTPCR Test

A. Screening gene:           E  

B. Internal Control gene: RPP 

C. Confirmatory gene:     ORF 

👉Usually, show the Sigmoid type of graph

👉The graph significantly rises only after 14 cycles which indicates CT. 

 

 🎯 RAPID TEST (TrueNat Test) :

 A. Screening gene:       E 

 B. Confirmatory gene: SARS CoviD 

💉Benefits: 

👉It useful in the emergency procedure 

     (e.g Perforation peritonitis) 

👉Shows result within just 45 min 

 💉Drawbacks: 

👉Sensitivity is poor as compared to RTPCR. 

👉Only 2 samples can be checked at a time. 

✨ RNA extraction & processing performed in 'Biosafety cabinet' where purified air with laminar flow is managed. 

✨RTPCR Plate later disinfected: By keeping them in 1% Hypochloride then autoclaving. 

 Later discarded as per BMW management protocol. 

🎯 BD BACTEC FX 40 :

external view of BD BACTEC

Showing samples placed inside the BD BACTEC

Monitor displaying result of sample.

💉Use: For detection of sample

💉 Result:

+ : Red

-: Green

Result pending: Orange 

💉Benefits: 

👉At a time 40 samples can be checked.

👉Unlike an incubator, it doesn't need 7 days, shows results within a day. 

 

🎯 BD Phoenix M50

BD Phoenix

💉Use :

👉Identification of organism. 

👉AST (Antibiotic Susceptibility Test).

💉Benefits: 

At a time 50 samples can be checked.

🎯 NEPHELOMETER

Nephelometer

💉Use :

👉To measure the turbidity of microbial suspension. 

FURTHER PART OF THIS MICROBIOLOGY PRACTICAL WILL BE COMPLETED IN FEW DAYS... DO VISIT AGAIN IF YOU WANT TO READ COMPLETELY....